Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2))

Immunocytochemistry/Immunofluorescence: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - IF assay in a high throughput format for screening Dengue virus inhibitors. HEK 293 cells in DMEM 5% FBS/1% PS at 5000 cells/well are infected in presence of inhibitors with Dengue 2 New Guinea (VR-1584) using 384-well collagen-coated plates. After 48 h incubation at 37C/5% CO2, the supernatant is removed and the Alexa Fluor 647 conjugated Ab is added at 1:5250. Plates were read after O/N incubation. Columns 1-2: Cells control. 23-24, rows A-L: Cells + virus control. 23-24, rows M-P: Cells + virus control + control drug (Positive control). 3-12 and 13-22: Experimental compounds added in a dose response manner from high to low concentration (Columns 3-13 higher concentration, columns 12-22 lower concentration). Image using the Alexa Fluor 647 format of this antibody.

Immunocytochemistry/Immunofluorescence: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - IF assay in a high throughput format for screening Dengue virus inhibitors. HEK 293 cells in DMEM 5% FBS/1% PS at 5000 cells/well are infected in presence of inhibitors with Dengue 2 New Guinea (VR-1584) using 384-well collagen-coated plates. After 48 h incubation at 37C/5% CO2, the supernatant is removed and the Alexa Fluor 647 conjugated Ab is added at 1:5250. Plates were read after O/N incubation. Columns 1-2: Cells control. 23-24, rows A-L: Cells + virus control. 23-24, rows M-P: Cells + virus control + control drug (Positive control). 3-12 and 13-22: Experimental compounds added in a dose response manner from high to low concentration (Columns 3-13 higher concentration, columns 12-22 lower concentration). Image using the Alexa Fluor 647 format of this antibody.

Immunocytochemistry/Immunofluorescence: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - IF assay in a high throughput format for screening Dengue virus inhibitors. HEK 293 cells in DMEM 5% FBS/1% PS at 5000 cells/well are infected in presence of inhibitors with Dengue 2 New Guinea (VR-1584) using 384-well collagen-coated plates. After 48 h incubation at 37C/5% CO2, the supernatant is removed and the Alexa Fluor 647 conjugated Ab is added at 1:5250. Plates were read after O/N incubation. Columns 1-2: Cells control. 23-24, rows A-L: Cells + virus control. 23-24, rows M-P: Cells + virus control + control drug (Positive control). 3-12 and 13-22: Experimental compounds added in a dose response manner from high to low concentration (Columns 3-13 higher concentration, columns 12-22 lower concentration). Image using the Alexa Fluor 647 format of this antibody.